Journal: Microorganisms

Article Title: Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis

doi: 10.3390/microorganisms9040728

Figure Lengend Snippet: Auto-aggregation ability, hydrophobicity, and adhesion ability to HeLa cells, and survivability in 0.01% H 2 O 2 of three candidate strains. (mean ± SD, n = 3).

Article Snippet: Accordingly, in the present study, we investigated the functions of two Lpb. plantarum strains ( Lpb. plantarum ZX27 and Lpb. plantarum ATCC 14917) and one commercial lactic acid bacteria (LAB) strain ( L. delbrueckii DM8909) and compared the probiotics based on their anti-adhesion, co-culture, and anti-biofilm activities against G. vaginalis .

Techniques:

Journal: Microorganisms

Article Title: Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis

doi: 10.3390/microorganisms9040728

Figure Lengend Snippet: ( A ) Co-aggregation of three Lactobacillus sp. with G. vaginalis. Inhibition rates of G. vaginalis in a co-culture assay with ( B ) L. delbrueckii DM8909, ( C ) Lpb. plantarum ATCC14917, and ( D ) Lpb. plantarum ZX27. The initial concentration of G. vaginalis was 10 4 CFU/mL. ( E ) Inhibition rate of G. vaginalis adhesion following pre-adhesion by Lactobacillus sp. (10 9 CFU/mL). The initial concentration of G. vaginalis was 10 7 CFU/mL. * p < 0.05. The data is represented as mean ± SD ( n = 3).

Article Snippet: Accordingly, in the present study, we investigated the functions of two Lpb. plantarum strains ( Lpb. plantarum ZX27 and Lpb. plantarum ATCC 14917) and one commercial lactic acid bacteria (LAB) strain ( L. delbrueckii DM8909) and compared the probiotics based on their anti-adhesion, co-culture, and anti-biofilm activities against G. vaginalis .

Techniques: Inhibition, Co-culture Assay, Concentration Assay

Journal: International journal of food microbiology

Article Title: Characterization of tannase activity in cell-free extracts of Lactobacillus plantarum CECT 748T.

doi: 10.1016/j.ijfoodmicro.2007.11.002

Figure Lengend Snippet: Fig. 1. HPLC analysis of tannic acid. (A) Modified basal media containing 1 mM tannic acid were inoculated with L. plantarum CECT 748T and incubated for 7 days at 30 °C (2); a noninoculated control medium was incubated in the same conditions (1). (B) L. plantarum CECT 748T cell-free extract incubated for 16 h in the presence of 1 mM tannic acid (2); phosphate buffer containing tannic acid was incubated in the same conditions and was used as control (1). Detection was performed at 280 nm.

Article Snippet: The type strain L. plantarum CECT 748T (ATCC 14917, DSMZ 20174) isolated from pickled cabbage was purchased from the Spanish Type Culture Collection.

Techniques: Modification, Incubation, Control

Journal: International journal of food microbiology

Article Title: Characterization of tannase activity in cell-free extracts of Lactobacillus plantarum CECT 748T.

doi: 10.1016/j.ijfoodmicro.2007.11.002

Figure Lengend Snippet: Fig. 2. Some biochemical properties of the L. plantarum CECT 748T tannase. (A) Optimum pH for activity. Tannase reactions were performed at pH ranging from 3 to 9. (B) Optimum temperature for tannase activity. Cell-free extracts were incubated at temperatures ranging from 20 to 70 °C. (C) Thermal stability of the tannase. Cell-free extracts were incubated at 22 °C (●), 37 °C (■), 45 °C (▲), or 55 (×) in phosphate buffer (50 mM, pH 6.5), at indicated times, aliquots were withdrawn, and analyzed as described in the Materials and methods section. The experiments were done in triplicate. The mean value and the standard error are showed.

Article Snippet: The type strain L. plantarum CECT 748T (ATCC 14917, DSMZ 20174) isolated from pickled cabbage was purchased from the Spanish Type Culture Collection.

Techniques: Activity Assay, Incubation

Journal: International journal of food microbiology

Article Title: Characterization of tannase activity in cell-free extracts of Lactobacillus plantarum CECT 748T.

doi: 10.1016/j.ijfoodmicro.2007.11.002

Figure Lengend Snippet: Fig. 3. Coomassie Blue (A) and tannase zymogram (B) of nondenaturing PAGE analysis of concentrated cell-free extracts from L. plantarum CECT 748T

Article Snippet: The type strain L. plantarum CECT 748T (ATCC 14917, DSMZ 20174) isolated from pickled cabbage was purchased from the Spanish Type Culture Collection.

Techniques:

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Initial rates of cadmium uptake by E. coli and L. plantarum in APT medium a

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques:

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Induction of the synthesis of mntA mRNA by Mn2+ starvation in L. plantarum ATCC 14917. Total RNA was isolated from L. plantarum ATCC 14917 Mn2+-starved (−) or Mn2+-sufficient (+) cells, treated with DNase I, and reverse transcribed with a specific antisense primer. The RT reaction mixture was used as a template to perform PCR to amplify a 1.1-kb fragment of mntA as described in the text. Each lane represents a separate experiment with four independent RNA preparations. MW, molecular weight.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Isolation, Reverse Transcription, Molecular Weight

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Identification of MntA in wild-type L. plantarum strains (A) and Mn2+-dependent mutants (B). Western blotting was done with an MntA antibody and protein extracts from L. plantarum strains and Cd2+ uptake mutants. (A) Lane 1, molecular mass standards (in kilodaltons); lanes 2 and 5, membrane protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 3 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 4 and 7, total protein from Mn2+-sufficient cells of ATCC 8014 and ATCC 14917, respectively. (B) Lanes 1 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 2 and 4, total protein from Mn2+-starved cells of mnd15-26 and mnd11-06, respectively; lanes 3 and 7, molecular weight standards; lane 5, membrane protein from Mn2+-starved cells of ATCC 8014.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Western Blot, Membrane, Molecular Weight